Kumar, R. and Arya, G. C. and Bisht, N. C. (2014) Differential Expression and Interaction Specificity of the Heterotrimeric G-Protein Family in Brassica nigra Reveal their Developmental- and Condition-Specific Roles. Plant and Cell Physiology, 55 (11). pp. 1954-1968.

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Abstract

Heterotrimeric G-proteins, comprised of alpha, beta and gamma subunits, are important signal transducers across phyla. The G-proteins are well characterized in the model plants Arabidopsis and rice, and their homologs are identified in a few other plant species; however, information about the roles played by G-proteins in regulating various growth and developmental traits particularly from polyploid crops is still awaited. In this study, we have isolated one G alpha (BniB.G alpha 1), three G beta (BniB.G beta 1-BniB.G beta 3) and four G gamma (BniB.G gamma 1-BniB.G gamma 4) coding sequences from the paleopolyploid Brassica nigra, a major condiment crop of the Brassicaceae family. Sequence and phylogenetic analysis revealed that whole-genome triplication events in the Brassica lineage had proportionally increased the inventory of the G beta subunit, but not of the G alpha and G gamma subunits in B. nigra. Real-time quantitative reverse transcription-PCR (qRT-PCR) analysis showed that members of the G-protein subunit genes have distinct temporal and spatial expression patterns and were differentially altered in response to various stress and phytohormone treatments, thereby suggesting differential transcriptional regulation of G-protein genes in B. nigra. Interestingly, specific members of G-protein subunits were co-expressed across plant developmental stages, and in response to different elicitor treatments. Yeast-based interaction screens further predicted that the B. nigra G-protein subunits interacted in most of the possible combinations, although showing a high degree of interaction specificity between different G-protein subunits. Our data on physical interactions coupled with the co-expression pattern of the multiple G-protein subunit genes suggested that tissue-and condition-specific functional combinations of G alpha beta gamma heterotrimers may exist in paleopolyploid B. nigra, to control diverse growth and development processes.

Item Type:	Article
Subjects:	Plant Genomics
Depositing User:	Unnamed user with email alok@urdip.res.in
Date Deposited:	10 Apr 2015 08:41
Last Modified:	10 Apr 2015 08:41
URI:	http://nipgr.sciencecentral.in/id/eprint/8

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