Agarwal, P. and Parida, S. K. and Mahto, A. (2014) Expanding frontiers in plant transcriptomics in aid of functional genomics and molecular breeding. Biotechnology Journal, 9 (12). pp. 1480-1492.

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Abstract

The transcript pool of a plant part, under any given condition, is a collection of mRNAs that will pave the way for a biochemical reaction of the plant to stimuli. Over the past decades, transcriptome study has advanced from Northern blotting to RNA sequencing (RNA-seq), through other techniques, of which real-time quantitative polymerase chain reaction (PCR) and microarray are the most significant ones. The questions being addressed by such studies have also matured from a solitary process to expression atlas and marker-assisted genetic enhancement. Not only genes and their networks involved in various developmental processes of plant parts have been elucidated, but also stress tolerant genes have been highlighted. The transcriptome of a plant with altered expression of a target gene has given information about the downstream genes. Marker information has been used for breeding improved varieties. Fortunately, the data generated by transcriptome analysis has been made freely available for ample utilization and comparison. The review discusses this wide variety of transcriptome data being generated in plants, which includes developmental stages, abiotic and biotic stress, effect of altered gene expression, as well as comparative transcriptomics, with a special emphasis on microarray and RNA-seq. Such data can be used to determine the regulatory gene networks, which can subsequently be utilized for generating improved plant varieties.

Item Type:	Article
Subjects:	Plant Genomics
Depositing User:	Unnamed user with email alok@urdip.res.in
Date Deposited:	09 Apr 2015 08:28
Last Modified:	09 Apr 2015 08:28
URI:	http://nipgr.sciencecentral.in/id/eprint/4

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