Irfan, M. and Ghosh, S. and Kumar, V. (2014) Insights into transcriptional regulation of beta-D-N-acetylhexosaminidase, an N-glycan-processing enzyme involved in ripening-associated fruit softening. JOurnal of Experimental Botany, 65 (20). pp. 5835-5848.

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Abstract

Tomato (Solanum lycopersicum) fruit ripening-specific N-glycan processing enzyme, beta-D-N-acetylhexosaminidase (beta-Hex), plays an important role in the ripening-associated fruit-softening process. However, the regulation of fruit ripening-specific expression of beta-Hex is not well understood. We have identified and functionally characterized the fruit ripening-specific promoter of beta-Hex and provided insights into its transcriptional regulation during fruit ripening. Our results demonstrate that RIPENING INHIBITOR (RIN), a global fruit ripening regulator, and ABSCISIC ACID STRESS RIPENING 1 (SlASR1), a poorly characterized ripening-related protein, are the transcriptional regulators of beta-Hex. Both RIN and SlASR1 directly bound to the beta-Hex promoter fragments containing CArG and C2-3(C/G)A cis-acting elements, the binding sites for RIN and SlASR1, respectively. Moreover, beta-Hex expression/promoter activity in tomato fruits was downregulated once expression of either RIN or SlASR1 was suppressed; indicating that RIN and SlASR1 positively regulate the transcription of beta-Hex during fruit ripening. Interestingly, RIN could also bind to the SlASR1 promoter, which contains several CArG cis-acting elements, and SlASR1 expression was suppressed in rin mutant fruits, indicating that RIN also acts as a positive regulator of SlASR1 expression during fruit ripening. Taken together, these results suggest that RIN, both directly and indirectly, through SlASR1, regulates the transcription of beta-Hex during fruit ripening. The fruit ripening-specific promoter of beta-Hex could be a useful tool in regulating gene expression during fruit ripening.

Item Type:	Article
Subjects:	Plant Genomics
Depositing User:	Unnamed user with email alok@urdip.res.in
Date Deposited:	10 Apr 2015 08:51
Last Modified:	10 Apr 2015 08:51
URI:	http://nipgr.sciencecentral.in/id/eprint/10

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